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Mammalian cell functions mediating recombination of genetic elements: topological restraints.

Authors
  • Upcroft, P
Type
Published Article
Journal
The Australian journal of experimental biology and medical science
Publication Date
Dec 01, 1986
Volume
64 ( Pt 6)
Pages
535–543
Identifiers
PMID: 3036053
Source
Medline
License
Unknown

Abstract

In extending the scope of our previous analyses of the functions that mammalian cells engender to recombine elements of genetic information (Upcroft, Carter and Kidson, 1980a and b), I describe the capacity of cells to recognise differentially combinations of open-ended and closed molecules. The frequency of successful recombination, as assayed by the generation of viable SV40 genomes from appropriate substrates, was shown to decrease with the loss of free termini capable of invasion of an homologous DNA duplex and to be influenced by the presence of non-homologous flanking sequences, the length of homology and whether the sequence was internal or terminal. The effect of stabilisation with long homologous regions on recombination over short homologous regions and inter-versus intra-molecular DNA sequence homologies was also investigated.

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