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Male meiotic spindle features that efficiently segregate paired and lagging chromosomes.

Authors
  • Fabig, Gunar1
  • Kiewisz, Robert1
  • Lindow, Norbert2
  • Powers, James A3
  • Cota, Vanessa4
  • Quintanilla, Luis J4
  • Brugués, Jan5
  • Prohaska, Steffen2
  • Chu, Diana S4
  • Müller-Reichert, Thomas1
  • 1 Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. , (Germany)
  • 2 Visualization and Data Analysis, Zuse Institute Berlin, Berlin, Germany. , (Germany)
  • 3 Light Microscopy Imaging Center, Indiana University, Bloomington, United States. , (India)
  • 4 Department of Biology, San Francisco State University, San Francisco, United States. , (United States)
  • 5 Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany. , (Germany)
Type
Published Article
Journal
eLife
Publisher
"eLife Sciences Organisation, Ltd."
Publication Date
Mar 09, 2020
Volume
9
Identifiers
DOI: 10.7554/eLife.50988
PMID: 32149606
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Chromosome segregation during male meiosis is tailored to rapidly generate multitudes of sperm. Little is known about mechanisms that efficiently partition chromosomes to produce sperm. Using live imaging and tomographic reconstructions of spermatocyte meiotic spindles in Caenorhabditis elegans, we find the lagging X chromosome, a distinctive feature of anaphase I in C. elegans males, is due to lack of chromosome pairing. The unpaired chromosome remains tethered to centrosomes by lengthening kinetochore microtubules, which are under tension, suggesting that a 'tug of war' reliably resolves lagging. We find spermatocytes exhibit simultaneous pole-to-chromosome shortening (anaphase A) and pole-to-pole elongation (anaphase B). Electron tomography unexpectedly revealed spermatocyte anaphase A does not stem solely from kinetochore microtubule shortening. Instead, movement of autosomes is largely driven by distance change between chromosomes, microtubules, and centrosomes upon tension release during anaphase. Overall, we define novel features that segregate both lagging and paired chromosomes for optimal sperm production. © 2020, Fabig et al.

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