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The major immediate-early genes of human cytomegalovirus induce two novel proteins with molecular weights of 91 and 102 kilodaltons.

Authors
  • Sadanari, H
  • Yamada, R
  • Yamagoshi, T
  • Ohnishi, K
  • Matsubara, K
  • Fukuda, S
  • Tanaka, J
Type
Published Article
Journal
Archives of virology
Publication Date
Jan 01, 2000
Volume
145
Issue
6
Pages
1257–1266
Identifiers
PMID: 10948998
Source
Medline
License
Unknown

Abstract

Mouse monoclonal antibody MAB810 is known to recognize the major immediate-early (IE) proteins, 68 kDa IE1 (IE1p68) and 80 kDa IE2 (IE2p80), of human cytomegalovirus (HCMV). Using this antibody we found that two additional proteins with higher molecular weights of approximately 91 (p91) and 102 kDa (p102) are also synthesized in HCMV-infected cells. p91 and p102 were produced in cells stably transfected with plasmid expressing IE1p68 and IE2p80, respectively, and shown to be related with IE1p68 and IE2p80, respectively, in primary amino acid sequence. Taken together, these results indicate that p91 and p102 are expressed from the IE1 and IE2 genes, respectively.

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