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MAIRA- real-time taxonomic and functional analysis of long reads on a laptop

Authors
  • Albrecht, Benjamin1
  • Bağcı, Caner1, 2
  • Huson, Daniel H.1
  • 1 University of Tübingen, Sand 14, Tübingen, Germany , Tübingen (Germany)
  • 2 Max Planck Institute for Developmental Biology and Eberhard Karls University Tübingen, Max-Planck-Ring 5, Tübingen, 72076, Germany , Tübingen (Germany)
Type
Published Article
Journal
BMC Bioinformatics
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 17, 2020
Volume
21
Issue
Suppl 13
Identifiers
DOI: 10.1186/s12859-020-03684-2
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundAdvances in mobile sequencing devices and laptop performance make metagenomic sequencing and analysis in the field a technologically feasible prospect. However, metagenomic analysis pipelines are usually designed to run on servers and in the cloud.ResultsMAIRA is a new standalone program for interactive taxonomic and functional analysis of long read metagenomic sequencing data on a laptop, without requiring external resources. The program performs fast, online, genus-level analysis, and on-demand, detailed taxonomic and functional analysis. It uses two levels of frame-shift-aware alignment of DNA reads against protein reference sequences, and then performs detailed analysis using a protein synteny graph.ConclusionsWe envision this software being used by researchers in the field, when access to servers or cloud facilities is difficult, or by individuals that do not routinely access such facilities, such as medical researchers, crop scientists, or teachers.

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