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Maintenance of Rhodopsin levels in Drosophila photoreceptor and phototransduction requires Protein Kinase D.

Authors
  • Ashe, Sudipta1, 2
  • Yadav, Shweta1
  • 1 a National Centre for Biological Sciences-TIFR, GKVK Campus, Bellary Road , Bangalore , 560065 , India. , (India)
  • 2 b Manipal Academy of Higher Education , Manipal , Karnataka , 576104 , India. , (India)
Type
Published Article
Journal
Fly
Publisher
Landes Bioscience
Publication Date
Jan 01, 2018
Volume
12
Issue
3-4
Pages
164–173
Identifiers
DOI: 10.1080/19336934.2019.1565256
PMID: 30663936
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

During Drosophila phototransduction, the G protein coupled receptor (GPCR) Rhodopsin (Rh1) transduces photon absorption into electrical signal via G-protein coupled activation of phospholipase C (PLC). Rh1 levels in the plasma membrane are critical for normal sensitivity to light. In this study, we report that Protein Kinase D (dPKD) regulates Rh1 homeostasis in adult photoreceptors. Although eye development and retinal structure are unaffected in the dPKD hypomorph (dPKDH), it exhibited elevated levels of Rh1. Surprisingly, despite having elevated levels of Rh1, no defect was observed in the electrical response to light in these flies. By contrast the levels of another transmembrane protein of the photoreceptor plasma membrane, Transient receptor potential (TRP) was not altered in dPKDH. Our results indicate that dPKD is dispensable for eye development but is required for maintaining Rh1 levels in adult photoreceptors.

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