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Macromolecular scaffolds for immobilizing small molecule microarrays in label-free detection of protein-ligand interactions on solid support.

Authors
  • Ys, Sun
  • Jp, Landry
  • Yy, Fei
  • Xd, Zhu
  • Jt, Luo
  • Xb, Wang
  • Kit S. Lam
Type
Published Article
Journal
Analytical Chemistry
Publisher
American Chemical Society
Volume
81
Issue
13
Pages
5373–5380
Identifiers
DOI: 10.1021/ac900889p
Source
Kit Lam Lab
License
Unknown

Abstract

We explored two macromolecular scaffolds, bovine serum albumin (BSA) and polyvinyl alcohol (PVA), as chemically complementary platforms for immobilizing small molecule compounds on functionalized glass slides. We conjugated biotin molecules to BSA and amine-derivatized PVA and subsequently immobilized the conjugates on epoxy-functionalized glass slides through reaction of free amine residues on BSA and PVA with surface-bound epoxy groups. We studied binding reactions of such immobilized small molecule targets with solution-phase protein probes using an oblique-incidence reflectivity difference scanning optical microscope. The results showed that both BSA and amine-derivatized PVA were effective and efficient as carriers of small molecules with NHS residues and fluoric residues and for immobilization on epoxy-coated solid surfaces. A significant fraction of the conjugated small molecules retain their innate chemical activity.

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