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Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?

Authors
  • Man, Weng Chi1
  • Ho, Jason Yen-Ping1
  • Wen, Yan1
  • Sokol, Eric R.1
  • Polan, Mary L.1
  • Chen, Bertha1
  • 1 Stanford University School of Medicine, Department of Obstetrics and Gynecology, 300 Pasteur Drive, HH-333, Stanford, CA, 94305-5317, USA , Stanford (United States)
Type
Published Article
Journal
International Urogynecology Journal
Publisher
Springer-Verlag
Publication Date
Sep 10, 2009
Volume
20
Issue
12
Pages
1423–1429
Identifiers
DOI: 10.1007/s00192-009-0905-y
Source
Springer Nature
Keywords
License
Yellow

Abstract

Introduction and hypothesisWe investigated whether the expression of alpha-1 antitrypsin (ATT), neutrophil elastase (NE), and lysyl oxidase-like protein 1 (LOXL-1) vary within the vagina in subjects with pelvic organ prolapse (POP).MethodsBiopsies were obtained from the anterior and posterior vaginal wall of 22 women with POP (≥stage 2 by POP-Q). The subjects were grouped by the most prominent defect: cystocele, cystocele plus uterine prolapse, and rectocele. Comparative real-time PCR, Western blotting, and NE enzyme activity assay were performed.ResultsThe ratio of anterior and posterior vaginal wall ATT, NE, and LOXL-1 expression varied between individuals within the same defect group.ConclusionsATT, NE, and LOXl-1 expression was variable among different biopsy sites in the vagina. No consistent pattern was present when the subjects were grouped by the most prominent defect. We recommend careful consideration of biopsy sites in future studies on POP to enhance reproducibility of data.

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