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Lysis of halobacteria in bacto-peptone by bile acids.

Authors
  • Kamekura, M
  • Oesterhelt, D
  • Wallace, R
  • Anderson, P
  • Kushner, D J
Type
Published Article
Journal
Applied and environmental microbiology
Publication Date
Apr 01, 1988
Volume
54
Issue
4
Pages
990–995
Identifiers
PMID: 16347619
Source
Medline
License
Unknown

Abstract

All tested strains of halophilic archaebacteria of the genera Halobacterium, Haloarcula, Haloferax, and Natronobacterium lysed in 1% Bacto-Peptone (Difco) containing 25% NaCl, whereas no lysis was observed with other strains belonging to archaebacteria of the genera Halococcus, Natronococcus, and Sulfolobus, methanogenic bacteria, and moderately halophilic eubacteria. Substances in Bacto-Peptone which caused lysis of halobacteria were purified and identified as taurocholic acid and glycocholic acid. High-performance liquid chromatography analyses of peptones revealed that Bacto-Peptone contained nine different bile acids, with a total content of 9.53 mg/g, whereas much lower amounts were found in Peptone Bacteriological Technical (Difco) and Oxoid Peptone. Different kinds of peptones can be used to distinguish halophilic eubacteria and archaebacteria in mixed cultures from hypersaline environments.

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