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Lysercell M enhances the detection of stage-specific Plasmodium-infected red blood cells in the automated hematology analyzer XN-31 prototype.

Authors
  • Toya, Yuji1
  • Tougan, Takahiro2
  • Horii, Toshihiro3
  • Uchihashi, Kinya4
  • 1 Cell Technology, Engineering 1, Sysmex Corporation, 4-4-4 Takatsukadai Nishiku, Kobe 651-2271, Japan. Electronic address: [email protected] , (Japan)
  • 2 Research Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: [email protected] , (Japan)
  • 3 Department of Malaria Vaccine Development, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: [email protected] , (Japan)
  • 4 Cell Technology, Engineering 1, Sysmex Corporation, 4-4-4 Takatsukadai Nishiku, Kobe 651-2271, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Parasitology international
Publication Date
Feb 01, 2021
Volume
80
Pages
102206–102206
Identifiers
DOI: 10.1016/j.parint.2020.102206
PMID: 33049417
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The automated hematology analyzers XN-30 (for research) and XN-31 prototype (for diagnosis support) can easily and rapidly detect Plasmodium-infected red blood cells (iRBCs) and distinguish the developmental stages of the parasite in approximately 1 min. Two dedicated reagents, Lysercell M and Fluorocell M, are available with the analyzers. Lysercell M plays an indispensable role in enhancing the fluorescence intensity of the nucleic acid staining dye in Fluorocell M and altering cell morphology. These effects of Lysercell M have been empirically determined but insufficiently analyzed. In this study, the properties of Lysercell M were analyzed using two flow cytometers and a fluorescence microscope. First, the fluorescence intensity emitted by iRBCs treated with Lysercell M or phosphate-buffered saline (PBS) was evaluated. Second, the size of RBCs treated with Lysercell M or PBS was measured. Finally, the morphology of individual parasites was observed after reconstruction of an M scattergram, a cytogram of the XN-31 prototype system, using an imaging flow cytometer. These analyses showed that treatment of iRBCs with Lysercell M increased the fluorescence intensity of stained parasite nucleic acids by approximately 10-fold and reduced the size of iRBCs in a stage-specific manner, facilitating the identification and quantification of ring form, trophozoite, and schizont stage iRBCs. These properties suggest that Lysercell M is useful for rapidly detecting iRBCs and accurately distinguishing the parasite developmental stages, thereby contributing to the usability of the XN-30 and XN-31 prototype analyzers. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

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