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Luffa operculata fruit aqueous extract induces motor impairments, anxiety-like behavior, and testis damage in rats.

Authors
  • Alves, Cinthia Dos S1
  • Frias, Humberto V1
  • Kirsten, Thiago B1
  • Cordeiro, Flora1
  • Bernardi, Maria M1
  • Suffredini, Ivana B2
  • 1 Graduation Program in Environmental and Experimental Pathology, Paulista University, R. Dr. Bacelar, 1212, Vila Clementino, São Paulo, SP 04026-002, Brazil. , (Brazil)
  • 2 Graduation Program in Environmental and Experimental Pathology, Paulista University, R. Dr. Bacelar, 1212, Vila Clementino, São Paulo, SP 04026-002, Brazil; Graduation Program in Dentistry and Center for Research in Biodiversity, Paulista University, Av. Paulista, 900, 1 andar, Cerqueira César, São Paulo, SP 01310-100, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Journal of ethnopharmacology
Publication Date
Aug 10, 2018
Volume
222
Pages
52–60
Identifiers
DOI: 10.1016/j.jep.2018.04.044
PMID: 29727732
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Luffa operculata (L.) Cogn., Cucurbitaceae (buchinha-do-norte), aqueous extract (EBN) is popularly used to relieve symptoms of sinusitis and as abortive. As neurotoxicity and toxicity studies on the male reproductive system are scarce, the present study aimed at quantitatively addressing the question. Male adult rats were observed in the open field (OF) and in the light-dark box test (LDB) to evaluate locomotion and anxiety. Macroscopical and microscopical alterations on the rats' testes were also studied. The rats were divided into two groups, control (GC) and experimental (GE). GE received 1.0 mg/kg per day of EBN, orally, for five consecutive days, whereas GC received water. On the 6th day, each animal was evaluated in OF and in LDB for 3 min in each apparatus. After that, the left testicles were studied. In the OF, GE showed decreased locomotion, increased immobility time and decreased grooming and remained for less time in the center of the apparatus. In LDB, GE showed significant difficulty in moving into the light side of the device and remained longer in the dark side, exhibiting less displacement on both sides and less transitions between sides. Testicle weights, relative weights, testicular volume, cranial-caudal and lateral-lateral axes presented an increase in relation to the GC. Microscopic changes were observed in parenchyma, lumen and diameter of seminiferous tubules. Leydig cell numbers were decreased in GE. The administration of EBN induced anxiety-like behavior, impaired locomotion and altered the testes morphology of rats. Copyright © 2018 Elsevier B.V. All rights reserved.

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