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Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells.

Authors
  • Dubyak, George R1
  • 1 Department of Physiology & Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Methods in enzymology
Publication Date
Jan 01, 2019
Volume
629
Pages
81–102
Identifiers
DOI: 10.1016/bs.mie.2019.10.006
PMID: 31727258
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The efficacy of cancer chemotherapy is enhanced by induction of sustainable anti-tumor immune responses. Such responses involve accumulation of immunogenic mediators, such as extracellular ATP and ATP metabolites, within the tumor microenvironment. Recent studies have identified nucleotide-permeable plasma membrane channels or pores that are activated as early downstream consequences of different regulated cell death pathways: pannexin-1 channels in apoptosis, MLKL pores in necroptosis, and gasdermin-family pores in pyroptosis. This chapter describes the use of highly quantitative and semi-high-throughput methods based on the ATP sensor luciferase to measure dynamic changes in extracellular ATP, ADP, and AMP in tissue/cell culture models of cancer cells during various modes of regulated cell death in response to chemotherapeutic drugs, death receptors, or metabolic perturbation. © 2019 Elsevier Inc. All rights reserved.

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