Affordable Access

Access to the full text

LOXL1-AS1 contributes to the proliferation and migration of laryngocarcinoma cells through miR-589-5p/TRAF6 axis

Authors
  • He, Guijun1
  • Yao, Wenfeng2
  • Li, Liang1
  • Wu, Yang1
  • Feng, Guojian1
  • Chen, Li3
  • 1 Lianyungang Second People’s Hospital, Lianyungang, Jiangsu, 222023, China , Lianyungang (China)
  • 2 The First People’s Hospital of Xinxiang City, Xinxiang, Henan, 453000, China , Xinxiang (China)
  • 3 Zaozhuang Municipal Hospital, No. 41, Longtou Middle Road, Shizhong District, Zaozhuang, Shandong, 277100, China , Zaozhuang (China)
Type
Published Article
Journal
Cancer Cell International
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Oct 13, 2020
Volume
20
Issue
1
Identifiers
DOI: 10.1186/s12935-020-01565-5
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundLOXL1-AS1 is a long non-coding RNA (lncRNA) that plays crucial roles in various cancers. However, the functional role of LOXL1-AS1 in laryngocarcinoma remains unclear. Thus we planned to probe into the function and underlying mechanism of LOXL1-AS1 in laryngocarcinoma.MethodsGene expression was evaluated in laryngocarcinoma cells using RT-qPCR. The ability of cell proliferation and migration was assessed by CCK8, colony formation, wound healing and transwell assays. The interaction among LOXL1-AS1, miR-589-5p and TRAF6 was detected by Ago2-RIP, RNA pull down and luciferase reporter assays.ResultsLOXL1-AS1 was overexpressed in laryngocarcinoma cells. Silencing of LOXL1-AS1 suppressed cell proliferation, migration and EMT in laryngocarcinoma. Moreover, miR-589-5p, the downstream of LOXL1-AS1, directly targeted TRAF6 in laryngocarcinoma. Importantly, LOXL1-AS1 augmented TRAF6 expression in laryngocarcinoma cells by sequestering miR-589-5p. Besides, miR-589-5p worked as a tumor-inhibitor while TRAF6 functioned as a tumor-facilitator in laryngocarcinoma. Of note, rescue experiments both in vitro and in vivo validated that LOXL1-AS1 aggravated the malignancy in laryngocarcinoma by targeting miR-589-5p/TRAF6 pathway.ConclusionsLOXL1-AS1 promotes the proliferation and migration of laryngocarcinoma cells through absorbing miR-589-5p to upregulate TRAF6 expression.

Report this publication

Statistics

Seen <100 times