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Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene.

Authors
  • Huang, T H
  • Quesenberry, J T
  • Martin, M B
  • Loy, T S
  • Diaz-Arias, A A
Type
Published Article
Journal
Diagnostic molecular pathology : the American journal of surgical pathology, part B
Publication Date
Jun 01, 1993
Volume
2
Issue
2
Pages
90–93
Identifiers
PMID: 8269282
Source
Medline
License
Unknown

Abstract

We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed microsatellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.

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