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Loss of endothelial cells in viral DNA-positive grafts after keratoplasty: a 2-year follow-up study.

Authors
  • Qu, Jing-Hao1, 2
  • Peng, Rong-Mei1, 2
  • Xiao, Ge-Ge1, 2
  • Qu, Hong-Qiang1, 2
  • Yu, Ting1, 2
  • Zhang, Shuang1, 2
  • Hong, Jing3, 2
  • 1 Department of Ophthalmology, Peking University Third Hospital, Beijing, China. , (China)
  • 2 Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, China. , (China)
  • 3 Department of Ophthalmology, Peking University Third Hospital, Beijing, China [email protected] , (China)
Type
Published Article
Journal
British Journal of Ophthalmology
Publisher
BMJ
Publication Date
Jan 01, 2022
Volume
106
Issue
1
Pages
26–31
Identifiers
DOI: 10.1136/bjophthalmol-2020-317629
PMID: 33055089
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To compare endothelial loss between recipients who received viral DNA-positive grafts and controls 2 years after corneal transplantation. We retrospectively analysed the clinical data and endothelial cell density of recipients of viral DNA-positive grafts and age-, sex-, aetiology- and operation-matched controls from April 2017 to July 2019 at the Peking University Third Hospital, Beijing, China. A total of 23/942 (2.44%) donor corneal buttons tested virus-positive by real-time PCR. A total of 27 recipients (except for 2 recipients) of viral DNA-positive grafts and 48 recipients of viral DNA-negative grafts were included in this study. Recipients of viral DNA-positive grafts had a higher endothelial cell (EC) loss rate post-penetrating keratoplasty and post-descemet stripping automated endothelial keratoplasty (p<0.05), but post-deep lamellar keratoplasty, the EC loss rate was similar to that of the controls. Recipients of herpes simplex virus-1-, cytomegalovirus- and varicella-zoster virus-positive grafts all had a higher EC loss rate than the controls during the 12- and 24-month follow-up periods (p<0.05). We inferred that viruses might be hidden in corneal grafts and mainly incubate in the corneal endothelium. Viral DNA-positive grafts do not need to be replaced immediately and can be followed up for a long time. © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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