The last past two decades, there has been a tremendous expansion in the discovery and description of novel insect-specific viruses (ISVs). This has corresponded with progresses in metagenomics analyses for virus revealing and the increasing attention in insect microbiomes. Plentiful of the new ISVs seem to be members of the family Flaviviridae, genus Flavivirus, and are usual in insect populations in wildlife, with a global geographic circulation. The gut microbiota of insects exhibits critical functions in the growth, expansion and environmental daptation to the host insects (1). Currently the insect microbiota and their microbiome, discovered from insects were recognized as major genetic resources for bio-processing industry. Accordingly, the development of insect gut microbiome by means of metagenomics advances will facilitate to discovery innovative biocatalysts and to improve novel strategies for recognizing smart molecules for biotechnological and medical purposes such as vaccines (1).