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The long-term oral exposure to titanium dioxide impaired immune functions and triggered cytotoxic and genotoxic impacts in rats.

Authors
  • Hashem, Mohamed M1
  • Abo-El-Sooud, Khaled1
  • Abd-Elhakim, Yasmina M2
  • Badr, Yahia Abdel-Hamid3
  • El-Metwally, Abeer E4
  • Bahy-El-Dien, Ahmed1
  • 1 Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. , (Egypt)
  • 2 Department of Forensic medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt. Electronic address: [email protected] , (Egypt)
  • 3 Department of Laser Sciences and interactions, National Institute of Laser Enhanced Sciences, Cairo University, Egypt. , (Egypt)
  • 4 Pathology Department, Animal Reproduction Research Institute, Giza, Egypt. , (Egypt)
Type
Published Article
Journal
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)
Publication Date
Feb 21, 2020
Volume
60
Pages
126473–126473
Identifiers
DOI: 10.1016/j.jtemb.2020.126473
PMID: 32142956
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Titanium dioxide "TiO2, E171″ is a widely used food additive that exists in various everyday food products all over the world together with vast applications in cosmetics and industry. However, many toxicological aspects particularly following oral exposure still unclear. Hence, this study was planned to examine the effect of oral exposure of male Wistar rats to two doses of TiO2 (20 or 40 mg/kg b.wt.) through oral gavage once daily for 90 consecutive days on the blood components, immunity, cytotoxic, and genotoxic indicators. A dose-dependent leukopenia, eosinophilia, neutrophilia, and thrombocytopenia were noted. Also, the immunoglobins G (IgG) and IgM were significantly elevated in TiO2 treated rats. The phagocytic activities, lysozyme, nitric oxide, and immunoglobulin levels were significantly depleted following TiO2 exposure. A significantly reduced lymphocyte proliferation but elevated LDH activity was prominent in TiO2 treated rats. Different pathological perturbations were observed in both splenic tissue and bone marrow. A marked increase in CD4+ and CD8+ immunolabeling was evident. A significant increase in the comet variables was recorded in response to the exposure of rats to the increasing level of TiO2 at both levels. Overall, these results indicated that TiO2 could induce hematotoxicity, genotoxic, and immunotoxic alterations with exposure for long durations. Copyright © 2020 Elsevier GmbH. All rights reserved.

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