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Long-term stabilized blood samples as controls for flow cytometric HLA-B27 screening: a feasibility study.

Authors
  • Levering, Wilfried H B M
  • Wind, Henk
  • Granger, Vivian
  • Sintnicolaas, Kees
  • Hooijkaas, Herbert
  • Reilly, John T
  • Gratama, Jan W
  • Barnett, David
Type
Published Article
Journal
Cytometry. Part B, Clinical cytometry
Publication Date
May 01, 2008
Volume
74
Issue
3
Pages
169–181
Identifiers
DOI: 10.1002/cyto.b.20388
PMID: 18200592
Source
Medline
License
Unknown

Abstract

Long-term stabilized whole blood samples are potentially useful as true HLA-B27 positive and true HLA-B27 negative control cells for daily and longitudinal quality control of flow cytometric HLA-B27 screening. In the same way, long-term stabilized samples may be used for EQA purposes. However, these samples are currently not feasible for reagent validation purposes. Extensive quality control of long-term stabilized samples is necessary before distribution in multicenter surveys.

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