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Logically derived sequence tandem mass spectrometry for structural determination of Galactose oligosaccharides.

Authors
  • Huang, Shih-Pei1
  • Hsu, Hsu Chen1
  • Liew, Chia Yen1, 2
  • Tsai, Shang-Ting1
  • Ni, Chi-Kung3, 4
  • 1 Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei, 10617, Taiwan. , (Taiwan)
  • 2 Molecular Science and Technology, Taiwan International Graduate Program, Academia Sinica and National Taiwan University, Taipei, 10617, Taiwan. , (Taiwan)
  • 3 Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei, 10617, Taiwan. [email protected] , (Taiwan)
  • 4 Department of Chemistry, National Tsing Hua University, Hsinchu, 30013, Taiwan. [email protected] , (Taiwan)
Type
Published Article
Journal
Glycoconjugate Journal
Publisher
Springer-Verlag
Publication Date
Apr 01, 2021
Volume
38
Issue
2
Pages
177–189
Identifiers
DOI: 10.1007/s10719-020-09915-1
PMID: 32062823
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Mass spectrometry has high sensitivity and is widely used in the identification of molecular structures, however, the structural determination of oligosaccharides through mass spectrometry is still challenging. A novel method, namely the logically derived sequence (LODES) tandem mass spectrometry (MSn), for the structural determination of underivatized oligosaccharides was developed. This method, which is based on the dissociation mechanisms, involves sequential low-energy collision-induced dissociation (CID) of sodium ion adducts, a logical sequence for identifying the structurally decisive product ions for subsequent CID, and a specially prepared disaccharide CID spectrum database. In this work, we reported the assignment of the specially prepared galactose disaccharide CID spectra. We used galactose trisaccharides and tetrasaccharides as examples to demonstrate LODES/MSn is a general method that can be used for the structural determination of hexose oligosaccharides. LODES/MSn has the potential to be extended to oligosaccharides containing other monosaccharides provided the dissociation mechanisms are understood and the corresponding disaccharide database is available.

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