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Locating intercalants within lipid bilayers using fluorescence quenching by bromophospholipids and iodophospholipids.

Authors
  • Alexenberg, Carmit1
  • Afri, Michal2
  • Eliyahu, Shlomi3
  • Porat, Hani4
  • Ranz, Ayala5
  • Frimer, Aryeh A6
  • 1 The Department of Chemistry, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: [email protected] , (Israel)
  • 2 The Department of Chemistry, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: [email protected] , (Israel)
  • 3 The Department of Chemistry, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: [email protected] , (Israel)
  • 4 The Department of Chemistry, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: [email protected] , (Israel)
  • 5 The Department of Chemistry, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: [email protected] , (Israel)
  • 6 The Department of Chemistry, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: [email protected] , (Israel)
Type
Published Article
Journal
Chemistry and physics of lipids
Publication Date
Jul 01, 2019
Volume
221
Pages
128–139
Identifiers
DOI: 10.1016/j.chemphyslip.2019.03.018
PMID: 30954536
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In previous work, we have been able to determine the depth of intercalated molecules within the lipid bilayer using the solvent polarity sensitivity of three spectroscopic techniques: the 13C NMR chemical shift (δ); the fluorescence emission wavelength (λem), and the ESR β-H splitting constants (aβ-H). In the present paper, we use the quenching by a heavy atom (Br or I), situated at a known location along a phospholipid chain, as a probe of the location of a fluorescent moiety. We have synthesized various phospholipids with bromine (or iodine) atoms substituted at various locations along the lipid chain. The latter halolipids were intercalated in turn with various fluorophores into DMPC liposomes, biomembranes and erythrocyte ghosts. The most effective fluorescence quenching occurs when the heavy atom location corresponds to that of the fluorophore. The results show that generally speaking the fluorophore intercalates the same depth independent of which lipid bilayer is used. KBr (or KI) is the most effective quencher when the fluorophore resides in or at the aqueous phase. Presumably because of iodine's larger radius and spin coupling constant, the iodine analogs are far less discriminating in the depth range it quenches. Copyright © 2019. Published by Elsevier B.V.

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