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Localization of plant lipids for light microscopy using p-phenylenediamine in tissues of Arachis hypogaea L.

Authors
  • Bal, A K
Type
Published Article
Journal
Stain technology
Publication Date
Jan 01, 1990
Volume
65
Issue
2
Pages
91–94
Identifiers
PMID: 1694315
Source
Medline
License
Unknown

Abstract

p-Phenylenediamine (pPD) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. Semithin plastic sections for light microscopy need no further staining and can be mounted in Histoclad after drying on a slide. Brown staining above background differentiates lipid-containing structures. Nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents.

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