Localization of plant lipids for light microscopy using p-phenylenediamine in tissues of Arachis hypogaea L.
- Published Article
- Publication Date
Jan 01, 1990
p-Phenylenediamine (pPD) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. Semithin plastic sections for light microscopy need no further staining and can be mounted in Histoclad after drying on a slide. Brown staining above background differentiates lipid-containing structures. Nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/1694315