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Localization of phospholipase Cbeta isozymes in the mouse cerebellum.

Authors
  • Sugiyama, T
  • Hirono, M
  • Suzuki, K
  • Nakamura, Y
  • Aiba, A
  • Nakamura, K
  • Nakao, K
  • Katsuki, M
  • Yoshioka, T
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Nov 19, 1999
Volume
265
Issue
2
Pages
473–478
Identifiers
PMID: 10558892
Source
Medline
License
Unknown

Abstract

To elucidate the role of phospholipase Cbeta (PLCbeta) isozymes in the cerebellum, the distributions of PLCbeta3 and PLCbeta4 were examined in wild-type and PLCbeta4-deficient mutant mice using immunohistochemistry, and the functions were evaluated by measurement of type 1 metabotropic glutamate receptor (mGluR1)-mediated inward current and Ca(2+) mobilization. In wild-type mice, PLCbeta4 was distributed equally in both rostral and caudal cerebellum, while PLCbeta3 was enriched in the caudal versus the rostral cerebellum. In PLCbeta4-deficient mice, there was no measurable inward current or intracellular Ca(2+) elevation in the rostral cerebellum, whereas small responses were observed in the caudal cerebellum. In wild-type mice, the inward current was observed only following the release of caged GTPgammaS, not caged IP(3). These results suggest that the signal transduction machinery, including receptors, G-proteins, PLCbeta3, PLCbeta4, and effectors, form a functional unit, and the deletion of PLCbeta4 alters this unit, markedly changing signal transduction efficacy.

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