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Localization of actin in normal human hepatocytes using fluorescent phallotoxins and immunohistochemical amplification.

Authors
  • Benkoel, L
  • Brisse, J
  • Capo, C
  • Benoliel, A M
  • Bongrand, P
  • Garcia, T
  • Chamlian, A
Type
Published Article
Journal
Cellular and molecular biology
Publication Date
Jul 01, 1992
Volume
38
Issue
4
Pages
377–383
Identifiers
PMID: 1499038
Source
Medline
License
Unknown

Abstract

Two different methods, fluorescent phallotoxins and immunohistochemical amplification systems were used to visualize actin in normal human hepatocytes. With fluorescent phallotoxins (NBD-phallacidin or rhodamine phalloidin), F-actin was distributed along the plasma membranes and at the bile canaliculi. With immunohistochemical methods (biotin-avidin, biotin-streptavidin, silver enhancement), actin was found at the same level, however a cytoplasmic staining was observed and discussed as G-actin localization.

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