Localization of actin in normal human hepatocytes using fluorescent phallotoxins and immunohistochemical amplification.
- Published Article
Cellular and molecular biology
- Publication Date
Jul 01, 1992
Two different methods, fluorescent phallotoxins and immunohistochemical amplification systems were used to visualize actin in normal human hepatocytes. With fluorescent phallotoxins (NBD-phallacidin or rhodamine phalloidin), F-actin was distributed along the plasma membranes and at the bile canaliculi. With immunohistochemical methods (biotin-avidin, biotin-streptavidin, silver enhancement), actin was found at the same level, however a cytoplasmic staining was observed and discussed as G-actin localization.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/1499038