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LncRNA and mRNA Expression Profiling in the Periurethral Vaginal Wall Tissues of Postmenopausal Women with Stress Urinary Incontinence.

Authors
  • Wei, Aimin1
  • Wang, Ruohan2
  • Wei, Kai3
  • Dai, Chencheng1
  • Huang, Yue3
  • Xu, Pengfei1
  • Xu, Juan1
  • Tang, Hui1
  • Zhang, Yan4
  • Fan, Yun5, 6
  • 1 Department of Gynecology and Obstetrics, The Affiliated Obstetrics and Gynecology Hospital, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China. , (China)
  • 2 The First Clinical Medical College, Nanjing Medical University, Nanjing, 211166, China. , (China)
  • 3 Kangda College of Nanjing Medical University, Lianyungang, 222002, China. , (China)
  • 4 Kangda College of Nanjing Medical University, Lianyungang, 222002, China. [email protected] , (China)
  • 5 State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, 211166, China. [email protected] , (China)
  • 6 Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166, China. [email protected] , (China)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Jul 01, 2020
Volume
27
Issue
7
Pages
1490–1501
Identifiers
DOI: 10.1007/s43032-020-00144-3
PMID: 32046467
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Stress urinary incontinence (SUI) is one of the major pelvic floor disorders affecting postmenopausal women. To investigate the lncRNA and mRNA expression profiling of SUI in postmenopausal women, we used a microarray analysis to examine the differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) in the periurethral vaginal wall of postmenopausal women with SUI. A total of 8840 lncRNAs and 7102 mRNAs were dysregulated in the two groups (absolute fold change ≥ 2 and P < 0.05). The expression levels of five randomly selected lncRNAs and mRNAs were validated by quantitative real-time PCR. A functional analysis revealed that several lncRNAs are involved in the lysosome pathway associated with extracellular matrix (ECM) remodeling. In addition, we also found several mRNAs involved in fibroblast pseudopodia formation, fibroblast growth, and the regulation of smooth muscle cell differentiation in the urinary tract. Our study offers essential data regarding differentially expressed lncRNAs and mRNAs and may provide new potential candidates for the study of SUI.

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