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LncRNA CASC15 Promotes Cerebral Ischemia/Reperfusion Injury via miR-338-3p/ETS1 Axis in Acute Ischemic Stroke

Authors
  • Chen, Chen1
  • Wang, Linjing1
  • Wang, Li2
  • Liu, Qi1
  • Wang, Chunying1
  • 1 Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, 150040
  • 2 Harbin Traditional Chinese Medicine Hospital, Harbin, 150076
Type
Published Article
Journal
International Journal of General Medicine
Publisher
Dove Medical Press
Publication Date
Oct 02, 2021
Volume
14
Pages
6305–6313
Identifiers
DOI: 10.2147/IJGM.S323237
PMCID: PMC8495001
Source
PubMed Central
Keywords
Disciplines
  • Original Research
License
Unknown

Abstract

Purpose Acute ischemic stroke (AIS) is a leading health problem caused by cerebral ischemia/reperfusion (CI/R). This study aimed to unveil the potential clinical value and mechanism of lncRNA CASC15. Patients and Methods The expression of CASC15, miR-338-3p was detected by quantitative real-time PCR (qRT-PCR). The correlations between CASC15 and national institutes of health stroke scale (NIHSS) scores or miR-338-3p were evaluated by Pearson correlation. A receiver operating characteristic (ROC) curve was performed to provide the diagnostic value of CASC15. Cell Counting Kit-8 (CCK-8) and flow cytometer were used to detect the condition of cell viability and apoptosis. The levels of interleukin-1beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) was detected by enzyme-linked immunosorbent assay (ELISA) assay. Results The expression of CASC15 was increased and the levels of miR-338-3p were decreased in AIS patients. A positive association between CASC15 and NIHSS score and an inverse association between CASC15 and miR-338-3p were revealed by Pearson correlation. CASC15 might discriminate AIS patients from healthy people. Silenced CASC15 exerted neuroprotective roles on cell viability, apoptosis, and inflammation via the miR-338-3p/ETS1 axis. Conclusion CASC15 might act as a potential diagnostic biomarker for AIS patients. CASC15/miR-338-3p/ETS1 axis played an essential role in cell viability, apoptosis, and neuroinflammation.

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