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Live Imaging of the Lung.

Authors
  • Brzoska, Tomasz1, 2
  • Kaminski, Tomasz W1
  • Bennewitz, Margaret F3
  • Sundd, Prithu1, 4, 5
  • 1 Pittsburgh Heart, Lung and Blood Vascular Medicine Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
  • 2 Division of Hematology/Oncology, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
  • 3 Department of Chemical and Biomedical Engineering, West Virginia University, Morgantown, West Virginia.
  • 4 Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania.
  • 5 Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Type
Published Article
Journal
Current protocols in cytometry
Publication Date
Dec 01, 2020
Volume
95
Issue
1
Identifiers
DOI: 10.1002/cpcy.80
PMID: 33226733
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion brought about by ventilation and cardiac contractions. A previous version of this Current Protocols in Cytometry article reported protocols for imaging ex vivo live lung slices and the intact mouse lung. Here, we update those protocols by adding new methodologies, new approaches for quantitative image analysis, and new areas of potential application. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Live imaging of lung slices Support Protocol 1: Staining lung sections with fluorescent antibodies Basic Protocol 2: Live imaging in the mouse lung Support Protocol 2: Intratracheal instillations Support Protocol 3: Intravascular instillations Support Protocol 4: Monitoring vital signs of the mouse during live lung imaging Support Protocol 5: Antibodies Support Protocol 6: Fluorescent reporter mice Basic Protocol 3: Quantification of neutrophil-platelet aggregation in pulmonary vasculature Basic Protocol 4: Quantification of platelet-dependent pulmonary thrombosis Basic Protocol 5: Quantification of pulmonary vascular permeability. © 2020 Wiley Periodicals LLC.

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