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Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation.

Authors
Type
Published Article
Journal
Nature Neuroscience
1097-6256
Publisher
Nature Publishing Group
Publication Date
Volume
16
Issue
5
Pages
580–586
Identifiers
DOI: 10.1038/nn.3371
PMID: 23542688
Source
Medline

Abstract

Astrocytes are thought to have important roles after brain injury, but their behavior has largely been inferred from postmortem analysis. To examine the mechanisms that recruit astrocytes to sites of injury, we used in vivo two-photon laser-scanning microscopy to follow the response of GFP-labeled astrocytes in the adult mouse cerebral cortex over several weeks after acute injury. Live imaging revealed a marked heterogeneity in the reaction of individual astrocytes, with one subset retaining their initial morphology, another directing their processes toward the lesion, and a distinct subset located at juxtavascular sites proliferating. Although no astrocytes actively migrated toward the injury site, selective proliferation of juxtavascular astrocytes was observed after the introduction of a lesion and was still the case, even though the extent was reduced, after astrocyte-specific deletion of the RhoGTPase Cdc42. Thus, astrocyte recruitment after injury relies solely on proliferation in a specific niche.

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