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Live-cell fluorescent biosensors for activated signaling proteins.

Authors
Type
Published Article
Journal
Current Opinion in Cell Biology
0955-0674
Publisher
Elsevier
Publication Date
Volume
14
Issue
2
Pages
167–172
Identifiers
PMID: 11891115
Source
Medline

Abstract

A new generation of live-cell fluorescent biosensors enables us to go beyond visualization of protein movements, to quantify the dynamics of many different protein activities. Alternate approaches can report post-translational modifications, ligand interactions and conformational changes, revealing how the location and subtle timing of protein activity controls cell behavior.

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