Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX.
Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK.
School of Physics & Astronomy, The University of Nottingham, University Park, Nottingham, UK; and School of Chemistry, University of Lincoln, UK.
Centre for Biomolecular Sciences, School of Pharmacy, The University of Nottingham, University Park, Nottingham, UK.
Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK. Electronic address: [email protected]
- Published Article
Nanomedicine : nanotechnology, biology, and medicine
- Publication Date
Aug 01, 2019
Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent. Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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This record was last updated on 12/31/2019 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/31048084