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Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX.

Authors
  • Al-Ani, Ali W1
  • Zhang, Lei1
  • Ferreira, Lenny1
  • Turyanska, Lyudmila2
  • Bradshaw, Tracey D3
  • Thomas, Neil R4
  • 1 Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK.
  • 2 School of Physics & Astronomy, The University of Nottingham, University Park, Nottingham, UK; and School of Chemistry, University of Lincoln, UK.
  • 3 Centre for Biomolecular Sciences, School of Pharmacy, The University of Nottingham, University Park, Nottingham, UK.
  • 4 Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK. Electronic address: [email protected]
Type
Published Article
Journal
Nanomedicine : nanotechnology, biology, and medicine
Publication Date
Aug 01, 2019
Volume
20
Pages
102005–102005
Identifiers
DOI: 10.1016/j.nano.2019.04.008
PMID: 31048084
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent. Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

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