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Liquid-chromatographic profiling of endogenous fluorescent substances in sera and urine of uremic and normal subjects.

Authors
  • Mabuchi, H
  • Nakahashi, H
Type
Published Article
Journal
Clinical chemistry
Publication Date
Apr 01, 1983
Volume
29
Issue
4
Pages
675–677
Identifiers
PMID: 6831696
Source
Medline
License
Unknown

Abstract

Endogenous fluorescent substances increase in serum during uremia. We have used "high-performance" liquid chromatography to profile these fluorescent substances in both uremic and normal body fluids. The fluorescence excitation and emission maxima we used were 322 and 415 nm, respectively. Of the numerous fluorescent substances found in uremic body fluids and in normal urine, some were also detectable in normal serum, but at relatively weak fluorescence intensities.

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