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Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules.

Authors
  • Homyak, Celia C
  • Fernandez, Ann
  • Touve, Mollie A1
  • Zhao, Bo
  • Anson, Francesca
  • Hardy, Jeanne A
  • Vachet, Richard W
  • Gianneschi, Nathan C1, 2
  • Ross, Jennifer L
  • Thayumanavan, S
  • 1 Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States. , (United States)
  • 2 Department of Chemistry and Biochemistry, University of California , San Diego, La Jolla, California 92093, United States. , (United States)
Type
Published Article
Journal
Biomacromolecules
Publisher
American Chemical Society
Publication Date
Jan 08, 2018
Volume
19
Issue
1
Pages
132–140
Identifiers
DOI: 10.1021/acs.biomac.7b01300
PMID: 29141403
Source
Medline
Language
English
License
Unknown

Abstract

Lipid-polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV-vis spectroscopy, fluorescence spectroscopy, and single-particle total internal reflection fluorescence (TIRF) microscopy. In addition, we confirmed that these dual-guest-loaded lipogels are stable in solution for several months. The simplicity of this complete aqueous formation and noncovalent dual-guest encapsulation holds potential as a tunable nanomaterial scaffold.

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