Affordable Access

Access to the full text

Lipidomic Phenotyping Reveals Extensive Lipid Remodeling during Adipogenesis in Human Adipocytes

Authors
  • Miehle, Florian1, 2
  • Möller, Gabriele1
  • Cecil, Alexander1
  • Lintelmann, Jutta1
  • Wabitsch, Martin
  • Tokarz, Janina1
  • Adamski, Jerzy1, 2, 3, 4
  • Haid, Mark1
  • 1 (J.A.)
  • 2 German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany
  • 3 Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, 85764 Neuherberg, Germany
  • 4 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore
Type
Published Article
Journal
Metabolites
Publisher
MDPI
Publication Date
May 26, 2020
Volume
10
Issue
6
Identifiers
DOI: 10.3390/metabo10060217
PMID: 32466532
PMCID: PMC7361991
Source
PubMed Central
Keywords
License
Green

Abstract

Differentiation of preadipocytes into mature adipocytes is a highly complex cellular process. At lipidome level, the adipogenesis remains poorly characterized. To investigate the lipidomic changes during human adipogenesis, we used the LipidyzerTM assay, which quantified 743 lipid species from 11 classes. The undifferentiated human SGBS cell strain showed a heterogeneous lipid class composition with the most abundant classes, phosphatidylethanolamines (PE), phosphatidylcholines (PC), and sphingomyelins (SM). The differentiation process was accompanied by increased ceramide concentrations. After completion of differentiation around day 4, massive lipid remodeling occurred during maturation, characterized by substantial synthesis of diacylglycerols (DAG), lysophosphatidylethanolamines (LPE), PC, PE, SM, and triacylglycerols (TAG). Lipid species composition became more homogeneous during differentiation to highly concentrated saturated and monounsaturated long-chain fatty acids (LCFA), with the four most abundant being C16:0, C16:1, C18:0, and C18:1. Simultaneously, the amount of polyunsaturated and very long-chain fatty acids (VLCFA) markedly decreased. High negative correlation coefficients between PE and PC species containing VLCFA and TAG species as well as between ceramides and SM imply that PE, PC, and ceramides might have served as additional sources for TAG and SM synthesis, respectively. These results highlight the enormous remodeling at the lipid level over several lipid classes during adipogenesis.

Report this publication

Statistics

Seen <100 times