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Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei.

Authors
  • Richmond, Gregory S1
  • Gibellini, Federica
  • Young, Simon A
  • Major, Louise
  • Denton, Helen
  • Lilley, Alison
  • Smith, Terry K
  • 1 Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
Type
Published Article
Journal
Parasitology
Publication Date
Aug 01, 2010
Volume
137
Issue
9
Pages
1357–1392
Identifiers
DOI: 10.1017/S0031182010000715
PMID: 20602846
Source
Medline
License
Unknown

Abstract

The biological membranes of Trypanosoma brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification of approximately 500 individual molecular phospholipids species from total lipid extracts of cultured bloodstream and procyclic form T. brucei. Various molecular species of all of the major subclasses of glycerophospholipids were identified including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol as well as phosphatidic acid, phosphatidylglycerol and cardolipin, and the sphingolipids sphingomyelin, inositol phosphoceramide and ethanolamine phosphoceramide. The lipidomic data obtained in this study will aid future biochemical phenotyping of either genetically or chemically manipulated commonly used bloodstream and procyclic strains of Trypanosoma brucei. Hopefully this will allow a greater understanding of the bizarre world of lipids in this important human pathogen.

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