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Lipid products of phosphoinositide 3-kinase abrogate genistein-induced fusion inhibition in myoblasts.

Authors
  • Woo, Joo Hong
  • Kim, Jeong Heon
  • Inhee, Mook-Jung
  • Kim, Hye Sun
Type
Published Article
Journal
European Journal of Pharmacology
Publisher
Elsevier
Publication Date
Jan 04, 2006
Volume
529
Issue
1-3
Pages
84–94
Identifiers
PMID: 16360147
Source
Medline
License
Unknown

Abstract

Genistein (4',5,7-trihydroxyisoflavone) is a tyrosine kinase inhibitor. Although the agent has shown to inhibit myoblast differentiation, neither intracellular target(s) as a tyrosine kinase inhibitor nor action mechanism of the agent is well known. Here we studied the effect of genistein on the differentiation of myoblasts. Genistein strongly but reversibly blocked both myoblast fusion and synthesis of the muscle-specific proteins. The agent also reversibly reduced the phosphorylation level of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase, and its interaction with p85, the regulatory subunit of phosphoinositide 3-kinase (PI3-kinase). In addition, genistein indirectly inhibited PI3-kinase activity and blocked calcium influx which is required for myoblast fusion. However, both genistein-induced inhibition of cell fusion and calcium influx were abrogated by the lipid products of PI3-kinase. These results demonstrate that genistein can exert their effect on the signaling pathway from FAK to calcium influx via PI3-kinase in the differentiation of myoblasts.

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