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LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression

Authors
  • Sun, Zhenxing1
  • Wei, Naili2
  • Yao, Shenglian3
  • Wang, Guihuai1
  • Sun, Yaxing4
  • Wang, Zhenze5
  • Yuan, Dan6
  • 1 Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, 102218, China , Beijing (China)
  • 2 The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, 515041, China , Shantou (China)
  • 3 University of Science and Technology Beijing, Beijing, China , Beijing (China)
  • 4 Zaozhuang Mental Health Center, Zaozhuang, Shandong, 277103, China , Zaozhuang (China)
  • 5 Haicheng Zhenggu Hospital, Anshan City, Liaoning, 114200, China , Anshan City (China)
  • 6 Beijing Luhe Hospital, Capital Medical University, No. 82, Xinhuanan Road, Tongzhou District, Beijing, 102218, China , Beijing (China)
Type
Published Article
Journal
Cancer Cell International
Publisher
Springer (Biomed Central Ltd.)
Publication Date
May 27, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12935-021-01931-x
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundLong non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated.MethodsqRT-PCR, western blot and GEPIA database were applied for reporting the expression of CENPK and LINC01158 in glioma and the correlation between LINC01158 and CENPK expression. EdU, colony formation, CCK-8, caspase-3 activity and TUNEL assays probed the impacts of LINC01158 on glioma cell growth. Subcellular fractionation and FISH assays revealed the cellular distribution of LINC01158. Luciferase reporter and RIP assays examined ceRNA network of LINC01158, CENPK and miR-6734-3p.ResultsLINC01158 and CENPK were both overexpressed in glioma and a positive regulation of LINC01158 on CENPK was corroborated. LINC01158 served a pro-proliferative and anti-apoptotic part in glioma by sponging miR-6734-3p to augment CENPK.ConclusionLINC01158 enhances CENPK by serving as sponge for miR-6734-3p to facilitate glioma development, proposing LINC01158 as a new player in glioma.

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