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Lentiviral vector-mediated siRNA knockdown of c-MYC: cell growth inhibition and cell cycle arrest at G2/M phase in Jijoye cells.

Authors
  • Song, Aiqin1
  • Ye, Junli
  • Zhang, Kunpeng
  • Sun, Lirong
  • Zhao, Yanxia
  • Yu, Hongsheng
  • 1 Department of Pediatric Hematology, Affiliated Hospital of Qingdao University Medical College, 16 Jiangsu Road, Qingdao, 266001 Shandong, China. [email protected] , (China)
Type
Published Article
Journal
Biochemical Genetics
Publisher
Springer-Verlag
Publication Date
Aug 01, 2013
Volume
51
Issue
7-8
Pages
603–617
Identifiers
DOI: 10.1007/s10528-013-9590-0
PMID: 23657834
Source
Medline
License
Unknown

Abstract

Inhibition of c-MYC has been considered as a potential therapy for lymphoma treatment. We explored a lentiviral vector-mediated small interfering RNA (siRNA) expression vector to stably reduce c-MYC expression in B cell line Jijoye cells and investigated the effects of c-MYC downregulation on cell growth, cell cycle, and apoptosis in vitro. The expression of c-MYC mRNA and protein levels were inhibited significantly by c-MYC siRNA. The c-MYC downregulation resulted in the inhibition of cell proliferation and cell cycle arrest at G2/M phase, which was associated with decreased expression of cyclin B and cyclin-dependent kinase 1 (CDK1) and increased expression of CDK inhibitor p21 proteins. In addition, downregulation of c-MYC induced cell apoptosis characterized by DNA fragmentation and caspase-3 activation. Taken together, these results suggest that lentiviral vector-mediated siRNA for c-MYC may be a promising approach for targeting c-MYC in the treatment of Burkitt lymphoma.

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