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LEAF-NODULE SYMBIOSIS I. : Endophyte of Psychotria Bacteriophila1

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Abstract

Centifanto, Ysolina M. (University of Florida, Gainesville), and Warren S. Silver. Leafnodule symbiosis. I. Endophyte of Psychotria bacteriophila. J. Bacteriol. 88:776–781. 1964.—The leaf-nodule endophyte of Psychotria bacteriophila has been repeatedly isolated in pure culture from germinating seedlings and young leaves on a nitrogen-free mineral agar. Its morphological, serological, and cultural characteristics place it within the Klebsiella-Aerobacter group. The endophyte has been provisionally named Klebsiella rubiacearum. Pure cultures fix N2 under anaerobic conditions with good efficiency (4.54 μg of N fixed/mg of glucose utilized in 3 days). Nitrogen fixation by pure cultures is intimately related to pyruvate and hydrogen metabolism.

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