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Lateral segregation of proteins induced by cholesterol in bacteriorhodopsin-phospholipid vesicles.

Authors
  • Cherry, R J
  • Müller, U
  • Holenstein, C
  • Heyn, M P
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Feb 15, 1980
Volume
596
Issue
1
Pages
145–151
Identifiers
PMID: 7353006
Source
Medline
Language
English
License
Unknown

Abstract

Bacteriorhodopsin in dimyristoylphosphatidylcholine vesicles is randomly distributed in the plane of the membrane and exhibits rotational diffusion above the gel to liquid-crystalline phase transition. Incorporation of cholesterol results in loss of rotational mobility of bacteriorhodopsin, which on the basis of electron microscopy and CD measurements can be assigned to the formation of protein aggregates. It is concluded that bacteriorhodopsin is soluble in the fluid phosphatidylcholine phase but segregates when cholesterol is present in the lipid bilayer.

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