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A lateral organ boundaries domain transcription factor acts downstream of the auxin response factor 2 to control nodulation and root architecture in Medicago truncatula

  • Kirolinko, Cristina
  • Hobecker, Karen
  • Cueva, Marianela
  • Botto, Florencia
  • Christ, Aurélie
  • Niebel, Andreas
  • Ariel, Federico
  • Blanco, Flavio Antonio
  • Crespi, Martín
  • Zanetti, María Eugenia
Publication Date
Apr 26, 2024
DOI: 10.1111/nph.19766
PMID: 38666352
OAI: oai:HAL:hal-04567150v1
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Summary Legume plants develop two types of root postembryonic organs, lateral roots and symbiotic nodules, using shared regulatory components. The module composed by the microRNA390, the Trans‐Acting SIRNA3 ( TAS3 ) RNA and the Auxin Response Factors (ARF)2, ARF3, and ARF4 (miR390/ TAS3 / ARFs ) mediates the control of both lateral roots and symbiotic nodules in legumes. Here, a transcriptomic approach identified a member of the Lateral Organ Boundaries Domain (LBD) family of transcription factors in Medicago truncatula , designated MtLBD17/29a , which is regulated by the miR390/ TAS3 / ARFs module. ChIP‐PCR experiments evidenced that MtARF2 binds to an Auxin Response Element present in the MtLBD17/29a promoter. MtLBD17/29a is expressed in root meristems, lateral root primordia, and noninfected cells of symbiotic nodules. Knockdown of MtLBD17/29a reduced the length of primary and lateral roots and enhanced lateral root formation, whereas overexpression of MtLBD17/29a produced the opposite phenotype. Interestingly, both knockdown and overexpression of MtLBD17/29a reduced nodule number and infection events and impaired the induction of the symbiotic genes Nodulation Signaling Pathway (NSP) 1 and 2 . Our results demonstrate that MtLBD17/29a is regulated by the miR390/ TAS3 / ARFs module and a direct target of MtARF2, revealing a new lateral root regulatory hub recruited by legumes to act in the root nodule symbiotic program.

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