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A laser scanning confocal microscopy method. Simultaneous detection of intracellular Ca2+ and apoptosis using Fluo-3 and Hoechst 33342.

Authors
  • Zhang, T
  • Cao, E H
  • Li, J F
Type
Published Article
Journal
Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology
Publication Date
Apr 01, 2000
Volume
22
Issue
2
Pages
93–97
Identifiers
PMID: 10800609
Source
Medline
License
Unknown

Abstract

We provided a useful method to exactly detect changes in intracellular Ca2+ in apoptotic cells, especially in single ones, by confocal microscopy and to exclude the artifact effect of necrotic and intact cells.

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