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Laser-Activated Gene Silencing via Gold Nanoshell-siRNA Conjugates.

Authors
  • Braun, Gary B
  • Pallaoro, Alessia
  • Wu, Guohui
  • Missirlis, Dimitris
  • Zasadzinski, Joseph A
  • Tirrell, Matthew
  • Reich, Norbert O
Type
Published Article
Journal
ACS Nano
Publisher
American Chemical Society
Publication Date
Jul 28, 2009
Volume
3
Issue
7
Pages
2007–2015
Identifiers
DOI: 10.1021/nn900469q
PMID: 19527019
Source
Medline
License
Unknown

Abstract

The temporal and spatial control over the delivery of materials such as siRNA into cells remains a significant technical challenge. We demonstrate the pulsed near-infrared (NIR) laser-dependent release of siRNA from coated 40 nm gold nanoshells. Tat-lipid coating mediates the cellular uptake of the nanomaterial at picomolar concentration, while spatiotemporal silencing of a reporter gene (green fluorescence protein) was studied using photomasking. The NIR laser-induced release of siRNA from the nanoshells is found to be power- and time-dependent, through surface-linker bond cleavage, while the escape of the siRNA from endosomes occurs above a critical pulse energy attributed to local heating and cavitation. NIR laser-controlled drug release from functional nanomaterials should facilitate more sophisticated developmental biology and therapeutic studies.

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