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Laser-Activated Corneal Adhesive: Retinal Safety in Rabbit Model

Authors
  • Tan, Jackie
  • Foster, Leslie John Ray
  • Lovicu, Frank James
  • Watson, Stephanie Louise
Type
Published Article
Journal
Translational Vision Science & Technology
Publisher
Association for Research in Vision and Ophthalmology (ARVO)
Publication Date
Jul 27, 2021
Volume
10
Issue
8
Identifiers
DOI: 10.1167/tvst.10.8.27
PMID: 34319386
PMCID: PMC8322714
Source
PubMed Central
Keywords
License
Unknown

Abstract

Purpose The purpose of this study was to investigate whether laser irradiation, used to activate an adhesive for sealing penetrating corneal incisions, causes any ophthalmoscopically or histologically visible retinal changes. Methods Baseline fundus assessment was conducted prior to laser irradiation of eyes of pigmented Dutch Belted rabbits. Treatment group was 18 eyes with the corneal adhesive activated in situ by a near infrared laser (125 mW for 45 seconds). The positive control group was 18 eyes, each irradiated for 60 seconds at 375, 500, 625, and 750 mW at different retinal locations. Unexposed regions of the retina were used as negative internal control. Ophthalmoscopic assessment was conducted immediately after laser exposure and prior to euthanasia. Retinas were histologically assessed at 0, 3, 72, and 168 hours after treatment. Results No ophthalmoscopically or histologically visible retinal changes were observed in the treatment group immediately, nor up to 168 hours after laser irradiation. In the positive control group, the incidences of ophthalmoscopically visible retinal lesions increased with irradiation power: 5.6% at 375 mW, 16.7% at 500 mW, 44.4% at 625 mW, and 50% at 750 mW. Histologically, retinal damage was observed as coagulative necrosis to all layers of the neural retina, including the retinal pigment epithelium. Conclusions The laser irradiation process used in the corneal adhesive technology did not cause any ophthalmoscopically or histologically visible retinal changes in the in vivo pigmented rabbit model. Prolonged exposure with this laser and at higher power can cause coagulative necrosis to the retina. Translational Relevance The corneal adhesive can be applied in humans without causing laser retinal damage.

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