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A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila.

Authors
  • Port, Fillip1
  • Strein, Claudia1
  • Stricker, Mona1
  • Rauscher, Benedikt1
  • Heigwer, Florian1
  • Zhou, Jun1
  • Beyersdörffer, Celine1
  • Frei, Jana1
  • Hess, Amy1
  • Kern, Katharina1
  • Lange, Laura1
  • Langner, Nora1
  • Malamud, Roberta1
  • Pavlović, Bojana1
  • Rädecke, Kristin1
  • Schmitt, Lukas1
  • Voos, Lukas1
  • Valentini, Erica1
  • Boutros, Michael1
  • 1 German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics and Heidelberg University, Heidelberg, Germany. , (Germany)
Type
Published Article
Journal
eLife
Publisher
"eLife Sciences Organisation, Ltd."
Publication Date
Feb 13, 2020
Volume
9
Identifiers
DOI: 10.7554/eLife.53865
PMID: 32053108
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Twenty years after the release of the sequence of the human genome, the role of many genes is still unknown. This is partly because some of these genes may only be active in specific types of cells or for short periods of time, which makes them difficult to study. A powerful way to gather information about human genes is to examine their equivalents in ‘model’ animals such as fruit flies. Researchers can use genetic methods to create strains of insects where genes are deactivated; evaluating the impact of these manipulations on the animals helps to understand the roles of the defunct genes. However, the current methods struggle to easily delete target genes, especially only in certain cells, or at precise times. Here, Port et al. genetically engineered flies that carry CRISPR-Cas9, a biological system that can be programmed to ‘cut’ and mutate precise genetic sequences. The insects were also manipulated in such a way that the CRISPR elements could be switched on at will, and their quantity finely tuned. This work resulted in a collection of more than 1,700 fruit fly strains in which specific genes could be deactivated on demand in precise cells. Further experiments confirmed that this CRISPR system could mutate target genes in different parts of the fly, including in the eyes, gut and wings. Port et al. have made their collection of genetically engineered fruit flies publically available, so that other researchers can use the strains in their experiments. The CRISPR technology they refined and developed may also lay the foundation for similar collections in other model organisms.

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