Affordable Access

Large-scale identification of protein-protein interaction of Escherichia coli K-12.

Authors
  • Arifuzzaman, Mohammad
  • Maeda, Maki
  • Itoh, Aya
  • Nishikata, Kensaku
  • Takita, Chiharu
  • Saito, Rintaro
  • Ara, Takeshi
  • Nakahigashi, Kenji
  • Huang, Hsuan-Cheng
  • Hirai, Aki
  • Tsuzuki, Kohei
  • Nakamura, Seira
  • Altaf-Ul-Amin, Mohammad
  • Oshima, Taku
  • Baba, Tomoya
  • Yamamoto, Natsuko
  • Kawamura, Tomoyo
  • Ioka-Nakamichi, Tomoko
  • Kitagawa, Masanari
  • Tomita, Masaru
  • And 3 more
Type
Published Article
Journal
Genome Research
Publisher
Cold Spring Harbor Laboratory
Publication Date
May 01, 2006
Volume
16
Issue
5
Pages
686–691
Identifiers
PMID: 16606699
Source
Medline
License
Unknown

Abstract

Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.

Report this publication

Statistics

Seen <100 times