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β-Lactotensin derived from bovine β-lactoglobulin exhibits anxiolytic-like activity as an agonist for neurotensin NTS(2) receptor via activation of dopamine D(1) receptor in mice.

Authors
  • Hou, I-Ching
  • Suzuki, Chihiro
  • Kanegawa, Norimasa
  • Oda, Ayako
  • Yamada, Ayako
  • Yoshikawa, Masaaki
  • Yamada, Daisuke
  • Sekiguchi, Masayuki
  • Wada, Etsuko
  • Wada, Keiji
  • Ohinata, Kousaku
Type
Published Article
Journal
Journal of Neurochemistry
Publisher
Wiley (Blackwell Publishing)
Publication Date
Nov 01, 2011
Volume
119
Issue
4
Pages
785–790
Identifiers
DOI: 10.1111/j.1471-4159.2011.07472.x
PMID: 21895659
Source
Medline
License
Unknown

Abstract

β-Lactotensin (His-Ile-Arg-Leu) is a bioactive peptide derived from bovine milk β-lactoglobulin, acting as a natural agonist for neurotensin receptors. We found that β-lactotensin exhibited anxiolytic-like activity in an elevated plus-maze test after its intraperitoneal (i.p.) administration in mice. β-Lactotensin was also orally active. The anxiolytic-like activity of β-lactotensin after i.p. administration was blocked by levocabastine, an antagonist for the neurotensin NTS(2) receptor. β-Lactotensin had anxiolytic-like activity in wild-type but not Ntsr2-knockout mice. β-Lactotensin increased intracellular Ca(2+) flux in glial cells derived from wild-type mice but not Ntsr2 knockout mice. These results suggest that β-lactotensin acts as an NTS(2) receptor agonist having anxiolytic-like activity. The anxiolytic-like activity of β-lactotensin was also blocked by SCH23390 and SKF83566, antagonists for dopamine D(1) receptor, but not by raclopride, an antagonist for D(2) receptor. Taken together, β-lactotensin may exhibit anxiolytic-like activity via NTS(2) receptor followed by D(1) receptor.

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