Bacillus sp. TS-23 amylase gene was amplified by polymerase chain reaction and cloned into the pET23(+) transcription vector. Lactose induction of Escherichia coli BL21(DE3) cells harbouring this recombinant plasmid resulted in the extracellular production of gene products. By the addition of 20 mmol l-1 lactose, the amylase activity of fermentation broth had a specific activity of 21 U mg-1 of protein.
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This record was last updated on 07/02/2016 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/9172444