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Lactose-induced expression of Bacillus sp. TS-23 amylase gene in Escherichia coli regulated by a T7 promoter.

Authors
  • Lin, L L
  • Hsu, W H
Type
Published Article
Journal
Letters in applied microbiology
Publication Date
May 01, 1997
Volume
24
Issue
5
Pages
365–368
Identifiers
PMID: 9172444
Source
Medline
License
Unknown

Abstract

Bacillus sp. TS-23 amylase gene was amplified by polymerase chain reaction and cloned into the pET23(+) transcription vector. Lactose induction of Escherichia coli BL21(DE3) cells harbouring this recombinant plasmid resulted in the extracellular production of gene products. By the addition of 20 mmol l-1 lactose, the amylase activity of fermentation broth had a specific activity of 21 U mg-1 of protein.

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