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Lactobacillus plantarum USM8613 Aids in Wound Healing and Suppresses Staphylococcus aureus Infection at Wound Sites.

Authors
  • Ong, Jia Sin1
  • Taylor, Todd D2, 3
  • Yong, Cheng Chung1
  • Khoo, Boon Yin4
  • Sasidharan, Sreenivasan4
  • Choi, Sy Bing5
  • Ohno, Hiroshi6, 7
  • Liong, Min Tze8, 9
  • 1 School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia. , (Malaysia)
  • 2 Laboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan. , (Japan)
  • 3 USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. , (Malaysia)
  • 4 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia. , (Malaysia)
  • 5 School of Data Sciences, Perdana University, 43400, Selangor, Malaysia. , (Malaysia)
  • 6 USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. [email protected] , (Malaysia)
  • 7 Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Yokohama, 230-0045, Japan. [email protected] , (Japan)
  • 8 School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia. [email protected] , (Malaysia)
  • 9 USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. [email protected] , (Malaysia)
Type
Published Article
Journal
Probiotics and antimicrobial proteins
Publication Date
Mar 01, 2020
Volume
12
Issue
1
Pages
125–137
Identifiers
DOI: 10.1007/s12602-018-9505-9
PMID: 30659503
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study aimed to elucidate the targets and mechanisms of anti-staphylococcal effects from bioactive metabolites produced by lactic acid bacteria. We aimed to better understand the safety and efficacy of these bioactive metabolites in in vivo systems, typically at topical sites. The cell-free supernatant and protein-rich fraction from Lactobacillus plantarum USM8613 inhibited staphyloxanthin biosynthesis, reduced (p < 0.05) the cell number of Staphylococcus aureus by 106 CFU/mL and reduced biofilm thickness by 55% in S. aureus-infected porcine skins. Genome-wide analysis and gene expression analysis illustrated the production of several plantaricins, especially the plantaricins EF and JK that enhanced the anti-staphylococcal effects of L. plantarum USM8613. In vivo data using rats showed that the protein-rich fraction from L. plantarum USM8613 exerted wound healing properties via direct inhibition of S. aureus and promoted innate immunity, in which the expression of β-defensin was significantly (p < 0.05) upregulated by 3.8-fold. The protein fraction from L. plantarum USM8613 also significantly enhanced (p < 0.05) the production of cytokines and chemokines through various stages of wound recovery. Using ∆atl S. aureus, the protein-rich fraction from L. plantarum USM8613 exerted inhibitory activity via targeting the atl gene in S. aureus. Taken altogether, our present study illustrates the potential of L. plantarum USM8613 in aiding wound healing, suppressing of S. aureus infection at wound sites and promoting host innate immunity.

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