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Lactic Acid Bacteria Improves Peyer's Patch Cell-Mediated Immunoglobulin A and Tight-Junction Expression in a Destructed Gut Microbial Environment.

Authors
  • Kim, Sung Hwan1
  • Jeung, Woonhee1
  • Choi, Il-Dong1
  • Jeong, Ji-Woong1
  • Lee, Dong Eun1
  • Huh, Chul-Sung2, 3
  • Kim, Geun-Bae4
  • Hong, Seong Soo5
  • Shim, Jae-Jung1, 2
  • Lee, Jung Lyoul1, 6
  • Sim, Jae-Hun1
  • Ahn, Young-Tae1
  • 1 R&BD Center, Korea Yakult Co. Ltd., Yongin 17086, Republic of Korea. , (North Korea)
  • 2 College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. , (North Korea)
  • 3 Research Institute of Eco-friendly Animal Sciences, Institute of Green Bio Science & Technology, Seoul National University, Pyeongchang 25354, Republic of Korea. , (North Korea)
  • 4 Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea. , (North Korea)
  • 5 Division of Gastroenterology, Vievisnamuh Hospital, Seoul 06117, Republic of Korea. , (North Korea)
  • 6 College of Veternary Medicine and Veternary Science Research Institute, Konkuk University, Seoul 05029, Republic of Korea. , (North Korea)
Type
Published Article
Journal
Journal of microbiology and biotechnology
Publication Date
Jun 28, 2016
Volume
26
Issue
6
Pages
1035–1045
Identifiers
DOI: 10.4014/jmb.1512.12002
PMID: 26975767
Source
Medline
Keywords
License
Unknown

Abstract

To evaluate the effects of lactic acid bacteria (LAB) on Peyer's patch cells, mice were treated with a high dose of kanamycin to disturb the gut microbial environment. The overarching goal was to explore the potential of LAB for use as a dietary probiotic that buffers the negative consequences of antibiotic treatment. In vitro, LAB stimulated the production of immunoglobulin A (IgA) from isolated Peyer's patch cells. Inflammation-related genes (TNF-α, IL-1β, and IL-8) were up-regulated in Caco-2 cells stimulated with lipopolysaccharide (LPS), while tight-junction-related genes (ZO-1 and occludin) were down-regulated; the effects of LPS on inflammatory gene and tight-junction gene expression were reversed by treatment with LAB. Mice treated with a high dose of kanamycin showed increased serum IgE levels and decreases in serum IgA and fecal IgA levels; the number of Peyer's patch cells decreased with kanamycin treatment. However, subsequent LAB treatment was effective in reducing the serum IgE level and recovering the serum IgA and fecal IgA levels, as well as the number of Peyer's patch cells. In addition, ZO-1 and occludin mRNA levels were up-regulated in the ileum tissues of mice receiving LAB treatment. Lactic acid bacteria can enhance the intestinal immune system by improving the integrity of the intestinal barrier and increasing the production of IgA in Peyer's patches. Lactic acid bacteria should be considered a potential probiotic candidate for improving intestinal immunity, particularly in mitigating the negative consequences of antibiotic use.

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