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Lack of synchrony among multiple nuclei induces partial DNA fragmentation in V79 cells polyploidized by demecolcine.

Authors
  • Fujikawa-Yamamoto, K
  • Ohdoi, C
  • Yamagishi, H
  • Zong, Z P
  • Murakami, M
  • Yamaguchi, N
Type
Published Article
Journal
Cell proliferation
Publication Date
Dec 01, 1999
Volume
32
Issue
6
Pages
337–349
Identifiers
PMID: 10646686
Source
Medline
License
Unknown

Abstract

The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K-252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidized by demecolcine but not by K-252a, which produced mononuclear cells. A lack of synchrony in the nuclear cycle was observed among nuclei in multinuclear polyploidized cells. Partial DNA fragmentation, defined as DNA fragmentation of a nucleus in a multinuclear cell, was detected using the TUNEL method in V79 cells polyploidized by demecolcine but not by K-252a. Apoptosis occurred earlier in cell populations treated with demecolcine than in these treated with K-252a once the drugs were removed from the medium, suggesting that polyploidized cells with separate nuclei tend to apoptose earlier than those with mononuclei.

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