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Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae.

Authors
  • Casal, M
  • Blázquez, M A
  • Gamo, F J
  • Gancedo, C
  • Leão, C
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
May 15, 1995
Volume
128
Issue
3
Pages
279–282
Identifiers
PMID: 7781975
Source
Medline
License
Unknown

Abstract

Mutants of Saccharomyces cerevisiae without phosphoenolpyruvate carboxykinase activity showed no measurable lactate proton symport, while mutants without fructose-1,6-bisphosphatase had normal transport activity. Incubation of a pck1 mutant, under derepression conditions in the presence of glycerol, restored the activity of the lactate-proton symport, with identical kinetic characteristics to that in the wild-type. For efficient lactate-proton symport activity, not only is an external inducer such as lactic acid needed, but also a molecule derived from the acid metabolism may be necessary.

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