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Lack of cross-reactivity of human and porcine reagents to quantify manatee (Trichechus manatus) cytokines.

Authors
  • Levin, Milton1
  • Jasperse, Lindsay2
  • Gebhard, Erika2
  • Rousselet, Estelle3
  • Walsh, Catherine4
  • 1 Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Unit 3089, Storrs, CT, 06269, United States. Electronic address: [email protected] , (United States)
  • 2 Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Unit 3089, Storrs, CT, 06269, United States. , (United States)
  • 3 Aquatic Animal Health Program, Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Florida, 2015 SW 16th Ave‬nue, Gainesville, FL, 32608, United States. , (United States)
  • 4 Marine Immunology Program, Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL, 34236, United States. , (United States)
Type
Published Article
Journal
Veterinary immunology and immunopathology
Publication Date
Sep 01, 2018
Volume
203
Pages
57–59
Identifiers
DOI: 10.1016/j.vetimm.2018.07.012
PMID: 30243374
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Veterinary medical examinations, including both physical examination and diagnostic tests, are important to monitor the health of both managed-care and wild marine mammals. However, limited species-specific reagents and assays are available that may contribute to a broader medical examination. This project evaluated if commercially available human and porcine antibodies and reagents would cross-react with manatee (Trichechus manatus) cytokines as the first step to validate a new diagnostic tool for manatees. Overall, as a result of limited cross-reactivity, human and porcine commercial reagents did not allow for the quantification of manatee cytokines. At this point, caution must be exercised when using human or porcine immunoassay reagents to quantify manatee cytokines if the reagents have not been fully validated. Future efforts will continue to explore and test the cross-reactivity of reagents to measure manatee cytokines as new species-specific and commercial reagents become available. Copyright © 2018 Elsevier B.V. All rights reserved.

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